Fig. 3: RNF8 deficiency promotes pro-inflammatory polarization of macrophages in the testicular microenvironment and led to spermatogenic dysfunction.

A The western blot and analysis of pro-Mφ (CD86, CD80, IL6) and anti-Mφ (CD163, CD206) marker protein level in the testis of WT and KO mice. B The mRNA of Ym1 and IL10 in the testis of WT and KO mice. C Multiple immunofluorescences staining of CD74, CD86, and CD163 in the testis of WT and KO groups, scale bar is 200 µm and 20 µm, respectively. D Changes of mRNA level of spermatogenic cells in different stages of mice testis in WT and KO groups, including Plzf, Grfα1, Id4, c-kit, and Scp3. E The western blot and analysis of Plzf, c-kit, and Scp3 in WT and KO groups. F Multiple immunofluorescences of Plzf, c-kit, and Scp3 in testicles of WT and KO, scale bar is 200 µm and 50 µm, respectively. The western blot (G) and immunofluorescence (H) were used to detect the expression of γ-H2AX in the testis of WT and KO, scale bar 20 µm. All values were presented as the mean ± SD. Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. WB strips in this figure correspond to Fig. 3 in WB's original data.