Fig. 4: RNF8 deficiency mice peritoneal macrophages hinders the development of spermatogenesis.

A The mRNA level of anti-Mφ (Arg1 and Retnla) markers in the peritoneal macrophages of WT and KO mice. B The protein level of anti-Mφ (CD206) and pro-Mφ (IL6 and CD86) markers in the peritoneal macrophages of WT and KO mice. C The mRNA level of IL6, NOS2, Ym1, Retnla, and Arg1 in the testis of WT + WT Mφ group and WT + KO Mφ group. D The protein level of CD86, CD163, and CD206 in the testis of WT + WT Mφ group and WT + KO Mφ group. E Multiple immunofluorescences staining of CD74, CD86, and CD163 in testicular tissues of the above two groups, scale bar is 200 µm and 20 µm, respectively. F The mRNA level spermatogenic cells in different stages (Grfα1, c-kit, and Scp3) of mice testis in the above two groups. G The protein level of spermatogenic cells in the testis of the above two groups, including Plzf, c-kit, and Scp3. H Multiple immunofluorescences of Plzf, c-kit, and Scp3 in the testis of the above two groups, scale bar is 200 µm and 20 µm, respectively. All values were presented as the mean ± SD. Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. WB strips in this figure correspond to Fig. 4 in WB's original data.