Fig. 8: RNF8 regulates the degradation of KDM6A through ubiquitination OPTN. | Cell Death Discovery

Fig. 8: RNF8 regulates the degradation of KDM6A through ubiquitination OPTN.

From: The RNF8/OPTN/KDM6A axis controls macrophage polarization to maintain testicular microenvironment homeostasis

Fig. 8

A Co-IP detection of the interaction between KDM6A and OPTN in cells. B, C Binding response (nm) between KDM6A and OPTN was measured by Octet R2 system. D, E 293T knocked down or overexpressed RNF8, IP-HA-OPTN, and detected the expression changes of KDM6A and LC3II. F GTEX database was used to analyze the correlation between the expression of OPTN and KDM6A in the testis. G Verification of OPTN knockout plasmid. H, I knocked down OPTN in 293T cells overexpressing RNF8 and detected the changes of KDM6A and LC3. J, K K448R was transfected into 293T cells overexpressing RNF8, and the changes of KDM6A and LC3 were detected. All values were presented as the mean ± SD. Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001.WB strips in this figure correspond to Fig. 8 in WB's original data.

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