Fig. 4: Analysis of SHMT activity and effect of UTR2 in HAP-1 cells.
From: RNA-mediated inhibition of mitochondrial SHMT2 impairs cancer cell proliferation
Trypan blue exclusion assay performed on HAP SHMT2KO complemented with SHMT2 WT (A) or with SHMT2 K281S-R284S (B), after 48 h of transfection with the indicated UTR2 sequences. Data shown as average ± SD from three replicates. n.s.: not significant *P < 0.05, **P < 0.001. Data from individual experiments were normalized to 100%, and subsequent values were calculated relative to this baseline before being averaged.
