Fig. 5: Effect of UTR2 expression on the survival of H1299 cells and in the mouse model.
From: RNA-mediated inhibition of mitochondrial SHMT2 impairs cancer cell proliferation
A Mitochondrial UTR2 expression: qRT-PCR on mitochondrial extracts of H1299 cells shows higher UTR2 expression in mitochondria when a mitochondrial RNA import signal is employed. Control involved cells transfected with an empty vector, showing low UTR2 expression. Data as average ± SD from three independent replicates. ***P < 0.0001. B Trypan blue exclusion assay carried out on H1299 cells lines stably transfected as indicated, 48 h after tetracycline treatment (0.06 µg/µl). Average ± standard deviation (SD) is shown. Data as average ± SD from three independent replicates. ***P < 0.0001. C Analysis (left panel) and images (right panel) of tumor volume 30 days after inoculation with the indicated cell lines and treatment with tetracycline in the drinking water. Average ± standard error (SR) is shown. The p-values (CTR vs mUTR2) for the last four statistically significant data points are 0.03, 0.05, 0.009, and 0.018, respectively.
