Fig. 6: Citrate supplementation exerts neuroprotective effects in PD mice. | Cell Death Discovery

Fig. 6: Citrate supplementation exerts neuroprotective effects in PD mice.

From: Mitochondrial dysfunction-mediated metabolic remodeling of TCA cycle promotes Parkinson’s disease through inhibition of H3K4me3 demethylation

Fig. 6

A Schematic diagram of the construction of MPTP-treated and citrate-supplemented mouse model. Time change curves of body weight (B), food intake (C) and water intake (D) during the establishment of the MPTP-treated mice receiving treatment with citrate, * shows the significant difference between this group and the Ctrl group. Data are mean ± SEM; n ≥ 5 biological replicates. E Organ-to-body weight ratios in the MPTP-treated mice receiving treatment with citrate. Data are mean ± SEM; n ≥ 5 biological replicates. F Serum and midbrain citrate levels in MPTP-treated mice receiving citrate treatment. The upper and lower boundary in the plot indicates the upper and lower quantiles, the line inside the plot the median; n = 6 biological replicates. Motor activity in the pole test (G) and inverted grid test (H) for MPTP-treated mice receiving treatment with citrate. Data are mean ± SEM; n = 8 biological replicates. I Representative fluorescence images (left) and fluorescence intensity analysis (right) of TH protein expression in the SN of the MPTP-treated mice receiving treatment with citrate. Data are mean ± SEM; n = 3 biological replicates. J Representative images of SNCA, H3K4me2, and H3K4me3 protein expression in the MPTP-treated mice receiving treatment with citrate. K Expression level analysis of SNCA protein expression in the MPTP-treated mice receiving treatment with citrate. Data are mean ± SEM; n = 4 biological replicates. α-KG levels (L), fumarate levels (M), and α-KG/fumarate ratio (N) in the citrate-supplemented MPTP mouse model. The upper and lower boundary in the plot indicates the upper and lower quantiles, the line inside the plot the median; n = 6 biological replicates. O Expression level analysis of H3K4me2 and H3K4me3 protein expression in the MPTP-treated mice receiving treatment with citrate. Data are mean ± SEM; n = 4 biological replicates. P Detection of the enrichment level of the Snca promoter fragment bound to H3K4me3 using CUT&RUN-qPCR. Data are mean ± SEM; n = 6 biological replicates.

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