Fig. 4: Intracerebroventricular administration of AMG487 preserves BBB integrity. | Cell Death Discovery

Fig. 4: Intracerebroventricular administration of AMG487 preserves BBB integrity.

From: Astrocyte-derived CXCL10 exacerbates endothelial cells pyroptosis and blood–brain barrier disruption via CXCR3/cGAS/AIM2 pathway after intracerebral hemorrhage

Fig. 4

A–F Quantification of CXCR3, cGAS, STING, ZO-1, claudin-5 and occludin relative mRNA level, n = 6. G Representative TEM images showing BBB disruption in perihematomal tissue at 24 h following ICH, n = 6. H Evans blue extravasation assay demonstrating dye leakage in perihematomal tissue at 24 h following ICH, n = 6. I Representative immunofluorescence images showing vascular endothelial cells (vWF, green) in perihematomal tissue at 24 h following ICH, n = 6. J Quantitative analysis of vWF fluorescence intensity. K–Q Representative western blot bands and quantitative analysis of cGAS, STING, AIM2, ZO-1, occludin, and claudin-5, n = 6. TEM scale bar =1 μm; Immunofluorescence scale bar =50 μm. *P < 0.05 vs. sham; #P < 0.05 vs. ICH + vehicle.

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