Fig. 2: GLUT3 enhances the chemosensitivity of GBM to TMZ and CAPE.
From: GLUT3 enhances chemosensitivity in glioblastoma by transporting temozolomide and capecitabine
A GLUT3 KD efficiency in U87-MG cell line. Cell viability (B) and IC50 values (C) of different nucleoside analog drugs were measured via CCK-8 in U87 cells with or without GLUT3 KD after treatment with various doses of drugs. D GLUT3 OE efficiency in U251-MG cell line. Cell viability (E) and IC50 values F of gemcitabine, capecitabine, and temozolomide were measured via CCK-8 in U251-MG cells with or without GLUT3 OE. G GLUT3 mRNA and protein levels in different GBM cell lines. H Cell viability and IC50 values of capecitabine (left panels) and temozolomide (right panels) were measured via CCK-8 in different GBM cell lines. I The correlation between the relative expression of GLUT3 and its chemotherapy resistance of capecitabine (left panel) and temozolomide (right panel) in different GBM cell lines. J Cell viability of capecitabine and temozolomide was measured via CCK-8 in U87 cells with or without GLUT1 KD. K Cell viability of capecitabine and temozolomide was measured via CCK-8 in U251-MG cells with or without GLUT1 OE. Data are presented by means ± SEM of (n = 3) biologically independent experiments. Independent-sample t-tests (C, D, F, J, K) or one-way ANOVA with Tukey’s post-hoc test A were used for statistical analysis by using SPSS 20 (IBM). Pearson’s correlation analysis determined the correlation between two variables (I). A p value < 0.05 was considered significant (*P < 0.05, **P < 0.01, ***P < 0.001).
