Fig. 4: OA indued mitochondrial injury in cultured MCECs.

MCECs were seeded overnight, and upon reaching sub-confluence, followed by treatment with different doses of OA for 6 h. A–H Effect of different doses of OA on mitochondrial functions, including basal respiration (B), maximal respiration (C), spare respiration capacity (D), ATP production (E), proton leak (F), non-mitochondrial respiration (G), and basal ECAR (H). All datasets were analyzed by the Kruskal–Wallis test with Dunn’s multiple-comparisons post hoc test. * vs 0, P < 0.05, n = 4. OCR, oxygen consumption rate, ECAR extracellular acidification rate.