Fig. 2: Zharp1-163 suppresses lipid ROS peroxidation to inhibit ferroptosis. | Cell Death Discovery

Fig. 2: Zharp1-163 suppresses lipid ROS peroxidation to inhibit ferroptosis.

From: Discovery of Zharp1-163 as a dual inhibitor of ferroptosis and necroptosis for the treatment of inflammatory disorders and kidney injury

Fig. 2

A The ROS levels in HT-1080 cells were measured after pretreatment with DMSO or Zharp1-163 for 2 h, followed by induction with RSL3 for 5 h. B, C The levels of CHAC1 and PTGS2 mRNA expression were measured in HT-1080 cells pretreated with DMSO or Zharp1-163 for 2 h, followed by induction with RSL3 (B, C). D DPPH scavenging rates were detected after treatment with the indicated concentrations of vitamin C or Zharp1-163. E HT-1080 cells were treated with DMSO or Zharp1-163 for 2 h prior to treatment with erastin for different durations. The cells were collected, and GPX4 expression was analyzed via Western blotting. F GSH levels in HT-1080 cells were analyzed after pretreatment with the indicated concentrations of Zharp1-163 for 2 h, followed by induction with erastin. G Representative fluorescence microscopy images of HT-1080 cells treated with DFO (100 μM) or Zharp1-163 (10 μM), as assessed by the FerroOrange testing assay. Scale bar, 500 μm. The data are presented as the mean ± SD of triplicate samples. ***P < 0.001; ****P < 0.0001.

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