Fig. 4: Function of miRNA-125a-5p overexpression in TGF-β/Smad pathway of activated HSC-T6 cells.

A WB was conducted to estimate TGF-β, TGFβR1 and p-Smad2/3 levels, B quantitative analysis of TGF-β, TGFβR1 and p-Smad2/3 protein levels, C immunofluorescence was employed to measure the expression of TGFβR1 and p-Smad2/3. **p < 0.05, ** p < 0.01.