Fig. 6: miR-125a-5p overexpression inhibits TGFβR1 in vitro and in vivo. | Cell Death Discovery

Fig. 6: miR-125a-5p overexpression inhibits TGFβR1 in vitro and in vivo.

From: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy

Fig. 6

A qRT-PCR was employed for analyzing miRNA-125a-5p expression within HSC-T6 and BRL-3A cells overexpressing miRNA-125a-5p, B qRT-PCR was conducted for evaluating TGFβR1 expression within both cell lines overexpressing miRNA-125a-5p, C WB was performed for exploring TGFβR1 level in both cell lines overexpressing miRNA-125a-5p, D quantitative analysis TGFβR1 protein level in both cell lines overexpressing miRNA-125a-5p, E immunofluorescence was used to evaluate TGFβR1 expression in HSC-T6 cells overexpressing miRNA-125a-5p, F WB was conducted detect TGFβR1 expression in mouse liver tissues overexpressing miRNA-125a-5p, G quantitative analysis TGFβR1 protein levels inside liver tissues in liver fibrosis mice overexpressing miRNA-125a-5p, H immunohistochemistry was employed to evaluate TGFβR1 expression inside liver tissues in liver fibrosis mice overexpressing miRNA-125a-5p. *p < 0.05, ** p < 0.01.

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