Fig. 2: Hepatocyte exosomal miRNAs mediate metabolic communication between organs. | Cell Death Discovery

Fig. 2: Hepatocyte exosomal miRNAs mediate metabolic communication between organs.

From: Cold-induced hepatocyte-derived exosomes activate brown adipose thermogenesis via miR-293-5p-mediated transcriptional reprogramming

Fig. 2

A The in vivo protocol involved the intravenous injection of AAV9-Ctrl KD hepExos and AAV9-YBX1 KD hepExos. The following parameters were assessed: B The body weight of the mice from protocol (A), with n = 5 mice per group. C Rectal temperature measurements were taken when the mice from protocol (A) were exposed to a 4 °C environment, with n = 5 mice per group. D The fat mass weight of the mice from protocol (A), with n = 5 mice per group. E Gene expression in BAT primary cells was analyzed using quantitative PCR, as described in protocol (A), with n = 3 independent experiments. F Gene expression in BAT was analyzed using quantitative PCR, as described in protocol (A), with n = 5 independent experiments. G UCP1 protein levels were analyzed in immortalized brown adipocytes, BAT primary cells, and BAT, with n = 3 independent experiments. H C57BL/6 J male mice underwent indirect calorimetry analysis in the basal state, with n = 5 mice per group. I Hematoxylin and eosin (H&E) staining and UCP1 protein expression were detected by immunohistochemistry in the mice from protocol (A) across different treatment groups. The scale bar for BAT is 0.2 mm.

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