Fig. 4: Nitroxoline does not inhibit the NF-κB pathway.
From: Nitroxoline is a novel inhibitor of NLRP3-dependent pyroptosis
A Immunblot presenting IkBα and phosphorylated IkBα (pIkBα) levels in LPS-primed THP-1 cells treated with 80 µM nitroxoline or solvent control. β-Actin served as loading control. B Corresponding bar plots presenting the pIκBα/IκBα ratio (n = 4). C Representative images of p65 nuclear translocation in PMA-differentiated THP-1 macrophages. LPS-primed Macrophages were treated with 80 µM nitroxoline or DMSO. Cells were imaged at 32 × magnification, with a scale bar of 100 µm. p65 is presented in cyan and nuclei in magenta. D Barplot depicting the nuclear mean p65 fluorescence intensity; acquisition parameters were unchanged between measurements (n = 4). Data are presented as mean + SD. Data were analyzed using Student’s t-test. *p < 0.05.
