Fig. 2: Osmolarity-dependent changes in cellular neighborhood and size distribution.
From: Non-uniform impact of extracellular osmotic variations at subcellular level
A–F Pie charts depicting the distribution of neighbor types for asymmetric (A, C, E) and symmetric (B, D, F) cells under hypertonic (A, B), isotonic (C, D), and hypotonic (E, F) conditions. The large pie charts represent the proportion of asymmetric neighbors (blue), symmetric neighbors (orange), and cells without neighbors (gray) within each treatment subset. The small pie charts provide a more detailed breakdown: the top-right chart quantifies the number of asymmetric neighbors, while the bottom-left chart quantifies the number of symmetric neighbors for a given treatment. The numbers (1-4) indicate the specific count of neighboring cells in each subset. This classification helps visualize how osmotic conditions influence cellular interactions and clustering patterns. G, H Show the distribution of normalized frequency of number of cells against TPN values in the three treatment conditions (G-without shape segregation and H-with shape segregation) (I) shows the bar graphs of average TPN values (mean ± SE) in hypertonic (n = 143 asymmetric, n = 432 symmetric), hypotonic (n = 166 asymmetric, n = 346 symmetric), and isotonic conditions (n = 165 asymmetric, n = 383 symmetric) (J) shows the circularity value plot of the two shape subsets in the three treatment conditions.
