Fig. 3: Quantitative spatial analysis of intracellular density reveals osmolarity-induced apico-basal polarity and uniformity.
From: Non-uniform impact of extracellular osmotic variations at subcellular level
A–C shows bar graphs depicting average APVs of DIC images of in asymmetric (solid bars) and symmetric (patterned bars) RAW264.7 cells along the z (A), y (B), and x (C) axes in hypertonic (black and gray bars, n = 143 asymmetric representing n = 432 symmetric, respectively), hypotonic (dark and light blue bars representing n = 166 asymmetric, n = 346 symmetric, respectively), and isotonic (dark and light-yellow bars representing n = 165 asymmetric, n = 383 symmetric, respectively) conditions (mean ± SE). D–I Show the distribution normalized frequency of the number of cells along APV values for each octant (color coding below Fig. H and I) in asymmetric (D, F, H) and symmetric (E, G, I) RAW264.7 cells in hypertonic (D, E), isotonic (F, G) and hypotonic (H, I) conditions.
