Fig. 3: Intratumoral heterogeneity of intestinal-type gastric cancer and 1C metabolism enrichment in CD44-high clusters of intestinal-type gastric cancer. | Cell Death Discovery

Fig. 3: Intratumoral heterogeneity of intestinal-type gastric cancer and 1C metabolism enrichment in CD44-high clusters of intestinal-type gastric cancer.

From: One-carbon metabolic pathway is a novel molecular signature for CD44-positive intestinal-type gastric cancer

Fig. 3

A Vlnplot of CD44 expression between the two subtypes and B each clonal samples. C Immunohistochemical experiments for CD44 staining were performed on each clonal samples from the two subtypes. Scale bars: 100 μm. D Immunocytochemistry of GC141 clonal sample organoids to show the expression of CD44 (Alexa488, green). DAPI (blue) was used for nuclear staining. E ‘CellChat’ chord diagram showing ligand-receptor pairs and their weights contributing to signalings among clonal samples of intestinal-type GC, GC141. F KEGG pathway enrichment analysis of identified DEGs in GC141_C2 compared to other clonal samples of GC141. G Dotplot showing pathway enrichment of ‘CD44-high’ and ‘CD44-low’ expressing clusters of GC141_C2. H KEGG pathway enrichment analysis of identified DEGs in ‘CD44-high’ expressing clusters compared to ‘CD44-low’ expressing clusters of GC141_C2. I Vlnplot displaying the expression of 1C metabolism-related genes between ‘CD44-high’ and ‘CD44-low’ clusters of GC141_C2. Statistical comparisons were performed using two-tailed Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns not significant).

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