Fig. 1: Depletion of LTβR induces p53-mediated senescence.
From: Reduction of lymphotoxin beta receptor induces cellular senescence via the MDMX-p53 pathway
A, B A375 cells were transfected with 100 nM of siControl (control siRNA) or siLTβR (LTβR siRNA), followed by 100 ng/ml Dox treatment for 48 h. Morphological changes, relative cell number (A), and confocal images of a senescence green probe (B) were analyzed. C, D B16F10WT (LTβR WT), and B16F10LTβR-KO (LTβR knockout) cells were treated with 100 ng/ml Dox for 48 h. Morphological changes and relative cell number (C), and confocal images of senescence green probe (D) were examined. E, F Western blot images of A375 and B16F10 cells showing the indicated proteins in LTβR-depleted cells. S.E. short exposure, L.E. long exposure. Band intensities of p53, p21 and MDM2 were measured using ImageJ and normalized to β-actin. Results are presented as the mean ± SD from three separate experiments. B, D Fluorescence intensities for relative senescence green probe were quantified by ImageJ, and data are shown as mean ± SD from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, using Fisher’s LSD post hoc test. n.s not significant.
