Fig. 2: Overexpression of SLC25A10 can promote the proliferation and migration of cervical cancer cells.
From: SLC25A10 promotes cisplatin resistance by inhibiting ferroptosis in cervical cancer
Lentiviruses were transfected into CaSki and HeLa cells, and a knockdown group (“SH1/SH2”, which represent two different sequences) and its control SH-NC were constructed; similarly, an overexpression group (OV) and its control OV-NC were also constructed. The mRNA and protein levels were detected, and protein levels were semiquantified (A–C). Cell viability was determined by CCK-8 assay. After SLC25A10 knockdown, cell viability was decreased significantly but was increased in the SLC25A10-overexpression group (D, E). A colony formation assay was used to assess cell proliferation. After SLC25A10 silencing, cell proliferation slowed, whereas cells in the SLC25A10-overexpression group formed more colonies (F, G). A quantitative measurement using a scratch test to detect cell migration revealed that SLC25A10 silencing slowed cell migration in vitro, whereas SLC25A10 upregulation increased cell migration compared with that in the control group (H, I). Compared with the control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale =200 µm. The data represent the means ± SDs.
