Fig. 1: Co-culture with adipocytes promotes the migration and invasion of TNBC cells. | Cell Death Discovery

Fig. 1: Co-culture with adipocytes promotes the migration and invasion of TNBC cells.

From: Adipocyte-derived IL6 and triple-negative breast cancer cell-derived CXCL1 co-activate STAT3/NF-κB pathway to mediate the crosstalk between adipocytes and triple-negative breast cancer cells

Fig. 1

A The schematic diagram of co-culture model construction. Adipocytes hADSC and 3T3-L1 cells were induced to differentiate into mature adipocytes after 12 days of maturation, adipocytes in the lower chamber and TNBC cells in the compartment were co-cultured for 3 days; B Oil red O staining of adipocytes. Mature adipocytes secrete large amounts of lipid droplets; C, D Co-culture with adipocytes promotes the migration ability of TNBC cells. MDA-MB-468 or MDA-MB-231 cells were co-cultured with adipocytes or single inoculation for 3 days, and the migration experiment was carried out. C Co-culture with human hADSC cells. D Co-culture with mouse 3T3-L1 cells; E, F Co-culture with adipocytes promotes the invasion ability of TNBC cells. MDA-MB-468 or MDA-MB-231 cells were co-cultured with adipocytes or single inoculation for 3 days, and the invasion experiment was carried out. E Co-culture with human hADSC cells. F Co-culture with mouse 3T3-L1 cells. Error bars represent means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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