Fig. 3: The analysis of the potential mechanism and the detection of the expression level of related genes and proteins in cocultured TNBC cells. | Cell Death Discovery

Fig. 3: The analysis of the potential mechanism and the detection of the expression level of related genes and proteins in cocultured TNBC cells.

From: Adipocyte-derived IL6 and triple-negative breast cancer cell-derived CXCL1 co-activate STAT3/NF-κB pathway to mediate the crosstalk between adipocytes and triple-negative breast cancer cells

Fig. 3

MDA-MB-468 and MDA-MB-231 of TNBC cells were co-cultured with hADSC cells or cultured separately. After 3 days, the cells were collected for cell sequencing, RT-qPCR, ELISA and Western blotting. A The bubble diagram of the potential mechanism of co-cultured MDA-MB-231 cells was analyzed in GSE114604 data set; B Vanne diagram of differential gene intersection in GSE114604, hADSC&MDA-MB-468, and 3T3-L1&MDA-MB-468 datasets; C 53 differential overlapping gene enrichment analysis circles; D–F Detection of the gene and protein expressions of CXCL1, CXCL2 and CXCL3 in TNBC cells co-cultured with hADSC cells; G Detection of the secretion of CXCL1 and CXCL2 protein in cell culture medium after co-culture with hADSC cells; H–J Detection of the expression of IL6 gene and protein and the secretion of IL6 protein in hADSC cells co-cultured with TNBC cells. K, L Detection of the expression of CXCR2 gene and protein in hADSC cells co-cultured with TNBC cells. M, N Detection of the expression of IL6R gene and protein in TNBC cells co-cultured with hADSC cells; O MDA-MB-468 and MDA-MB-231 cells were cultured alone or co-cultured with hADSC cells. Add inhibitors for IL6R (Tocilizumab, 1 μM) and/or CXCR2 inhibitors (Navarixin, 1 nM), or PBS to the culture medium. 3 days later, detection of the expression of CXCL1 in TNBC cells and IL6 in hADSC cells.

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