Fig. 4: hADSC and TNBC cells co-cultured to activate STAT3/NF-κB p65 signal pathway in TNBC cells and hADSC cells. | Cell Death Discovery

Fig. 4: hADSC and TNBC cells co-cultured to activate STAT3/NF-κB p65 signal pathway in TNBC cells and hADSC cells.

From: Adipocyte-derived IL6 and triple-negative breast cancer cell-derived CXCL1 co-activate STAT3/NF-κB pathway to mediate the crosstalk between adipocytes and triple-negative breast cancer cells

Fig. 4

MDA-MB-468 and MDA-MB-231 of TNBC cells were co-cultured with hADSC cells or cultured separately. After 3 days, the cells were collected for Western blotting and cell immunofluorescence to detect the expression of related pathway proteins. A Western blotting was used to detect the expression of proteins related to STAT3/NF-κB p65 pathway in co-cultured TNBC cells; B–D The expression of proteins related to STAT3/NF-κB p65 pathway in co-cultured TNBC cells was detected by cellular immunofluorescence, MDA-MB-468 and MDA-MB-231 cells grew on the cover slides of the insert, respectively. The cells in the lower chamber were cultured alone or co-cultured with adipocytes. After 3 days, the cells were fixed and stained, and the expressions of P-JAK2, P-STAT3 and P-NF-κB p65 were detected. The nucleus was stained with DAPI. Scale bars, 20 μm; E MDA-MB-468 and MDA-MB-231 cells were cultured alone or co-cultured with hADSC cells. Add inhibitors for IL6R (Tocilizumab, 1 μM) and/or CXCR2 inhibitors (Navarixin, 1 nM), or PBS to the culture medium. 3 days later, TNBC cells were collected to detect the expression of proteins related to STAT3/NF-κB p65 pathway; F MDA-MB-468 and MDA-MB-231 cells were cultured alone or co-cultured with hADSC cells. Add inhibitors for IL6R (Tocilizumab, 1 μM) and/or CXCR2 inhibitors (Navarixin, 1 nM), and PBS to the culture medium. 3 days later, hADSC cells were collected to detect the expression of proteins related to STAT3/NF-κB p65 pathway.

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