Fig. 6: Diet-induced obese model mouse increased tumor-bearing load. | Cell Death Discovery

Fig. 6: Diet-induced obese model mouse increased tumor-bearing load.

From: Adipocyte-derived IL6 and triple-negative breast cancer cell-derived CXCL1 co-activate STAT3/NF-κB pathway to mediate the crosstalk between adipocytes and triple-negative breast cancer cells

Fig. 6

A Female C57BL/6J mouse of HFD-induced obesity model were fed with high-fat diet or controlled diet for 10–12 weeks, and a total of 4 × 106 MDA-MB-468 cells/200 μL was injected into each C57BL/6J mouse fat pad for 10–14 days; B The body weight of HFD mouse increased significantly; C, D The tumor growth rate and tumor load of HFD mouse was faster than that of CD mouse; E, F Detection of the secretion levels of serum CXCL1 and IL6 of mouse after tumor bearing; G Western blotting was used to detect the expression of CXCL1 in tumor tissue and IL6 in adipose tissue; H–J Western blotting was used to detect the expression of proteins related to STAT3/NF-κB p65 pathway in tumor tissue and adipose tissue; J Western blotting was used to detect the expression of MMP7/MMP9 in tumor tissue of DIO and CD mouse.

Back to article page