Fig. 3: TRIM21 regulated DNA replication in CRC cells.
From: TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis
A Fluorescence detection of EdU-positive cells in CRC cells with TRIM21 knockdown or overexpression. Nuclei were stained with DAPI (blue), and replicating DNA was incorporated with EdU (red). Scale bars = 50 μm. **P < 0.01, ***P < 0.001, ****P < 0.0001. B Detection of EdU-positive cells in CRC cells with TRIM21 knockdown or overexpression by EdU/PI double staining assay. **P < 0.01. C Cell cycle analysis of HCT8 cells collected at different time points (0 h, 2 h, 4 h, 6 h, 8 h) after release of synchronization from siNC group and siTRIM21 groups by flow cytometry. D Detection of EdU-positive cells in HCT8 cells collected at different time points (0 h, 2 h, 4 h, 6 h, 8 h) after release of synchronization from siNC group and siTRIM21 groups by EdU/PI double staining assay. E Results of DNA fiber assay from HCT8 cells with or without TRIM21 knockdown. The representative images were shown in the left panel. Green fluorescence represented IdU incorporated DNA fibers and red fluorescence represented CIdU incorporated DNA fibers. Scale bars = 5 μm. Analysis of replication fork velocity was presented in the right panel. ****P < 0.0001.
