Fig. 1: High expression of Fra-1 in gastric cancer correlates with poor prognosis and enhanced cancer cell properties.

A Box plot showing the expression levels of Fra-1 in the gastric cancer cohort (STAD) from the TCGA database. “Normal” refers to gastric glandular epithelium, while “Tumor” refers to gastric cancer tissue. FDR (False Discovery Rate) = 5.1e-01. B Kaplan–Meier overall survival curve analysis based on Fra-1 expression levels in gastric cancer patients from the GEO dataset. The graph illustrates the survival rates of patients with high Fra-1 expression versus those with low Fra-1 expression at various time points. Hazard Ratio (HR) = 1.57 (95% CI: 1.33–1.87), log-rank p-value = 1.5e-07. C GEPIA2 database analysis of Fra-1 expression levels across different clinical stages of gastric cancer. F value = 1.31, Pr (>F) = 0.27. D Representative immunohistochemical images showed the expression of Fra-1 in gastric cancer tissues and corresponding paracancerous tissues. 20×: 50 μm; 40×: 20 μm. E Immunohistochemical semi-quantitative statistical chart. Normal: 21 cases; Cancer: 23 cases; The p-value was calculated through unpaired t-test correction. F, G Overexpression of Fra-1 in gastric cancer cells HGC27/AGS was achieved, and an EdU cell proliferation assay combined with flow cytometry was utilized to assess changes in the proliferation ability of gastric cancer cells. The p-value was calculated through unpaired t-test correction. H, I Knockdown of Fra-1 in gastric cancer cells HGC27/AGS was performed, and the effect on cell proliferation was measured using an EdU cell proliferation assay combined with flow cytometry analysis. The p-value was calculated through unpaired t-test correction. J, K The effect of Fra-1 knockdown on the invasion ability of gastric cancer cells HGC27/AGS was determined using a Transwell cell invasion assay. Scale bar, 100 μm. The p-value was calculated through unpaired t-test correction. L, M A scratch healing assay was conducted to investigate the impact of Fra-1 on the migration ability of gastric cancer cells HGC27/AGS. Scale bar, 200 μm. The p-value was calculated through unpaired t-test correction. All experiments were independently repeated three or more times, and the data presented are from representative individual experiments. “ns” indicates no significant difference; “*“ indicates p < 0.05; “**” indicates p < 0.01; “***” indicates p < 0.001; “****” indicates p < 0.0001.