Fig. 5: Impact of COL1 treatment of ovarian cancer cells on PARPi cytotoxicity. | Cell Death Discovery

Fig. 5: Impact of COL1 treatment of ovarian cancer cells on PARPi cytotoxicity.

From: Increased efficacy of PARP inhibitors against cisplatin-sensitive and -resistant ovarian cancer cells mediated via ATR and ATM inhibition

Fig. 5

A Ratio of cDDP, niraparib and olaparib LC50 values between ovarian cells cultivated with and without COL1. This is shown for W1, W1CR, A2780 and A2780cis cells. Data represent means ± CI (n = 6). B Representative curve of live apoptosis detection in W1 cells upon the indicated treatments for 72 h. C Representative curve of live necrosis detection in W1 cells upon the indicated treatments for 72 h. D Histograms showing the slope of the initial linear part of the curves quantifying the kinetic of apoptosis induction. E Histograms showing the time required to reach half of the maximum fluorescence value. Both, apoptosis and necrosis were measured in parallel in the indicated cell lines treated with either 5 µM (W1), 15 µM (W1CR, A2780) or 40 µM (A2780cis) of the respective PARPi according to the cell viability data in Fig. 1. Mean ± SD (n = 3), for statistical analysis One-way ANOVA following Tukey’s test was performed, *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001.

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