Fig. 6: Ly6C+Ly6G+CD4+ T cells promote macrophage polarization to M1 state after APP infection. | Cell Death Discovery

Fig. 6: Ly6C+Ly6G+CD4+ T cells promote macrophage polarization to M1 state after APP infection.

From: IL-21-dependent Ly6C+Ly6G+CD4+ T cells found in lung enhance macrophages function against Actinobacillus pleuropneumoniae infection in mice

Fig. 6

A–E RAW264.7 macrophages were co-cultured with IL-21, Ly6C+Ly6G+CD4+ T cells, and Ly6C+Ly6G-CD4+ T cells for 84 h. A The microscopic morphology of each group of cells (under 40x microscope, scale bar=100 μm). B CCK8 experiments detect the proliferation of RAW264.7 macrophages. C–E ELISA assays detect the content of (C) Arginase I, (D) IL-18, and (E) TNF-α in the cell culture supernatant. F–J After co-culture, RAW264.7 macrophages were stimulated with APP (MOI = 5) for 30 min, the gentamicin was then added to remove extracellular bacteria. After that, the cells were cultured for 6 h to detect the macrophage polarization. F–I The representative gating strategies and percentage of CD86+ and CD206+ cells detected by flow cytometry. J The content of TNF-α in the culture supernatant. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, using Mann-Whitney U test. Error bars show means ± SEM.

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