Fig. 5: Immunofluorescence analysis of GABRG2 expression and localization in Gabrg2+/A105T mice. | Cell Death Discovery

Fig. 5: Immunofluorescence analysis of GABRG2 expression and localization in Gabrg2+/A105T mice.

From: A knock-in mouse model for GABRG2-related epileptic encephalopathy displays spontaneous generalized seizures and cognitive impairment

Fig. 5

Immunofluorescence staining of GABRG2 (green) and β-tubulin III (red) in the hippocampal CA1 (A), CA3 (B), and DG (C) regions of Gabrg2+/A105T and WT mice. GABRG2 was predominantly localized on the neuronal membrane, with some cytoplasmic expression. Scale bars = 500 µm for the first column and = 20 µm for the rest. D Average fluorescence intensity of GABRG2 in the hippocampal CA1, CA3, and DG regions of Gabrg2+/A105T and WT mice (n = 3 per group). E Nissl staining of hippocampal regions in Gabrg2+/A105T and WT mice. Scale bars = 500 µm. F Quantification of number of neurons in the hippocampus. Data are presented as mean ± SD and * P < 0.05 vs. WT using two-way ANOVA followed by Sidak’s multiple comparisons test (5D) or Unpaired t-test (5F).

Back to article page