Fig. 2: Analysis of transcriptome and glycolytic features of cabozantinib-resistant cells. | Cell Death Discovery

Fig. 2: Analysis of transcriptome and glycolytic features of cabozantinib-resistant cells.

From: Modulating metabolic signatures to mitigate cabozantinib resistance in FLT3-ITD acute myeloid leukemia cell models

Fig. 2

A Enrichment plot from the gene-set enrichment analysis (GSEA). The normalized enrichment scores (NES) of indicated pathways are calculated based on log (Fold Change) *log(p-value) between MV4-11-XR and MV4-11. B Volcano plot showing DEGs that are upregulated (red dots) or downregulated (blue dots) of resistant cells compared to parental cells. DEGs related to the KEGG metabolic pathway were shown as yellow dots. C Seahorse Glycolysis Stress Test was performed to examine basal glycolysis, glycolytic capacity, and glycolytic reserve of indicated cells. D, E Glucose consumption, lactate production, and GAPDH activity of indicated cells. F RT-qPCR analysis of indicated genes in Molm13, Molm13-XR, MV4-11, and MV4-11-XR cells; 18 s rRNA was used for normalization. Data are representative of two independent experiments each performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 compared with parental cells.

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