Fig. 7: Loss of Asxl1 dysregulates transcriptional program through RNAPII transcriptional activity.
From: Loss of ASXL1 in the bone marrow niche dysregulates hematopoietic stem and progenitor cell fates
a Heatmaps of ASXL1 and RNAPII on ASXL1-bound genes in WT and Asxl1−/− BMSCs. The genes ranked from highest to lowest ASXL1 level. b Asxl1 deletion reduces genome-wide RNAPII occupancy. c RNAPII traveling ratio (TR) shows that many genes become less paused in Asxl1−/− BMSCs compared with WT controls. Lower TR values indicate a lower degree of pausing. d Average genome-wide occupancies of RNAPII in WT (blue) and Asxl1−/− (red) BMSCs on downregulated genes along the transcription unit. e RNAPII TR calculations of downregulated genes in WT and Asxl1−/− BMSCs. f, g Representative genome browser tracks showing ASXL1 and RNAPII occupancies on regions of Cxcl1, Cxcl2, and Vcam1
