Fig. 2: Repopulated microglia replenish the entire retina after removal of CSF1R inhibition.
From: Dual extra-retinal origins of microglia in the model of retinal microglia repopulation
a Scheme of microglial repopulation and time points for examination. b Spatial distributions of retinal microglia show that microglia repopulate the whole retina after removal of PLX5622. Each green dot represents a microglial cell. c Zoom-in images of microglia in the OPL. Green: GFP; magenta: BrdU. d Quantification of microglial density in the OPL during microglial repopulation. The red line and pink area indicate the mean and SD of microglial density in normal retinas, respectively. NS not significant; *p < 0.05 to D0; **p < 0.01 to D0; ***p < 0.001 to D0; #p < 0.05 to the normal retina; ##p < 0.01 to the normal retina; ###p < 0.001 to the normal retina. e Quantification of BrdU-positive microglia among all microglia in the OPL during repopulation. NS not significant; *p < 0.05 to D0; **p < 0.01 to D0; ***p < 0.001 to D0. S: superior; I: inferior; N: nasal; T: temporal; PLX5622: PLX5622 formulated diet; CD: control diet. Arrows: BrdU + microglia. The data are presented as mean ± SD. One-way ANOVA with Bonferroni’s post hoc
