Fig. 6: Periphery-emerging microglia are derived from macrophages in the ciliary body/iris.
From: Dual extra-retinal origins of microglia in the model of retinal microglia repopulation
a Scheme of genetic inducible fate mapping for the ciliary body and iris macrophage derived microglia in repopulated retinas. b Confocal images show that all macrophages in the ciliary body/iris (left) and all repopulated microglia (right) in the peripheral retina are labeled with tdTomato. c Scheme of ex vivo culture of retinal explants with or without the ciliary body and iris. d Confocal images show that repopulated microglia emerge in the ciliary body and iris-connected retinal explants after ex vivo culture for 11 days (left), but not in the ciliary body and iris-removed retinal explants (right). PLX5622: PLX5622 formulated diet; CD: control diet. Cyan: Iba1; red: tdTomato; green: Iba1 (b) or GFP (d); magenta: Iba1; blue: DAPI
