Fig. 2: Characterization of ZIKV-infected cells in the fetal astrocyte cultures. | Cell Discovery

Fig. 2: Characterization of ZIKV-infected cells in the fetal astrocyte cultures.

From: Zika virus propagation and release in human fetal astrocytes can be suppressed by neutral sphingomyelinase-2 inhibitor GW4869

Fig. 2

Human fetal astrocytes were mock-infected or infected with ZIKV strains MR766 or PRVABC59 at the MOI of 0.5 for 24 h, washed, then cultured for another 24 h. a–c At the experimental endpoint, ICC of SOX2 (purple color), GFAP (red color), and flavivirus antigen (green color) was performed. The white arrow, double arrow, and arrowhead denotes bipolar-shaped GFAP-positive, and fibrous GFAP-positive, and GFAP-negative ZIKV-infected cells, respectively. d–f ICC of TuJ1 (purple color), GFAP (red color), and flavivirus antigen (green color) was performed. g–i ICC of S100B (red color) and flavivirus antigen (green color) was performed. DAPI (blue color) was used as a nuclear counterstain for all ICC imaging. Panels are representative of three separate donors. Images were acquired through a Zeiss LSM 710 confocal microscope. Scale bar: 50 μm. CTL control

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