Fig. 4: ZKV induces apoptosis in astrocytes. | Cell Discovery

Fig. 4: ZKV induces apoptosis in astrocytes.

From: Zika virus propagation and release in human fetal astrocytes can be suppressed by neutral sphingomyelinase-2 inhibitor GW4869

Fig. 4

Human fetal astrocytes were infected with ZIKV strains MR766 or PRVABC59 at the MOI of 0.5 for 24 h, washed, then cultured for another 24 h. a–c ICC of activated caspase 3 (red color) and flavivirus antigen (green color) and was performed. DAPI (blue color) was used as a nuclear counterstain. The white arrowhead and arrow denotes apoptotic infected astrocyte and apoptotic uninfected astrocyte, respectively. Scale bar: 50 μm. d ZIKV- and activated caspase 3-dual-positive cells in a–I were quantified and shown as percentage of total cells in the cultures. Ten random fields from each group were manually counted by a blinded assessor. CTL control. e Cells positive with activated caspase 3 were broken down between ZIKV-positive and ZIKV-negative groups. Results are normalized as percentage of total activated caspase 3-positive cells and are representative of three independent experiments. **p < 0.01, ***p < 0.001, in comparison to mock-infected control (ANOVA, N = 10)

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