Fig. 3: Recombinant mutant flagellin of Salmonella typhi (SF) without its C-terminal 35 amino acids fails to activate NLRC4 pathway. | Cell Discovery

Fig. 3: Recombinant mutant flagellin of Salmonella typhi (SF) without its C-terminal 35 amino acids fails to activate NLRC4 pathway.

From: Sequence determinants of specific pattern-recognition of bacterial ligands by the NAIP–NLRC4 inflammasome

Fig. 3

a Caspase-1 activation detected 1 h after transfection of 100 nM flagellins in LPS pretreated BMMs. b IL-1β secretion detected 20 h after transfection of 100 nM flagellins in LPS pretreated BMMs. c Co-immunoprecipitation assay of flagellin of E. coli K12 strain (KF), flagellin of Salmonella typhi (SF) and C35 deletion variant of SF with NAIP5 in 293T cells. d Co-immunoprecipitation assay of flagellins KF, SF, and C35 replacement variant of SF with NAIP5 in 293T cells. Shown are immunoblots of anti-Flag immunoprecipitates (IP: Flag) and total cell lysates (Input)

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