Fig. 6: abLIM1 binds to and organizes F-actin into dense networks in vitro. | Cell Discovery

Fig. 6: abLIM1 binds to and organizes F-actin into dense networks in vitro.

From: abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing

Fig. 6

a Diagrams of human abLIM1 and its mutants used for in vitro assays. LIM, LIM motifs; DHU, dematin-homologous unfolded region; VHP, villin headpiece domain. b Proteins expressed and purified from E. coli. Bovine serum albumin (BSA) was loaded for quantification. All the lanes were from the same gel. c Experiment scheme. See Methods for details. d ΔLIM induced F-actin network formation by binding to F-actin. Note that the VHP domain alone neither bound to F-actin nor led to actin network formation. e Negative staining EM indicated that ΔLIM crosslinked and bundled F-actin

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