Fig. 7: The dematin-homologous region of abLIM1 binds to both cortex proteins and cortical actin. | Cell Discovery

Fig. 7: The dematin-homologous region of abLIM1 binds to both cortex proteins and cortical actin.

From: abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing

Fig. 7

a Diagrams of human abLIM1 and its mutants. LIM, LIM motif-containing region; DHU, dematin-homologous unfolded region; VHP, villin headpiece domain. b, c abLIM1 associated with cell cortex proteins spectrins and adducin through the dematin-homologous region. The indicated Flag-tagged (b) or GFP-tagged (c) proteins were expressed in HEK293T cells and subjected to co-IP. Exogenous luciferase (Luc) and endogenous GAPDH served as negative controls. All the lanes in (c) were from same blots. d Subcellular localization of GFP-tagged abLIM1 mutants. RPE1 cells were transfected for 24 h to express the indicated proteins and then stained for F-actin. e Model. In non-erythroid cells such as RPE1 and U2OS, the cortex is composed of a layer of spectrin network (only a few components are illustrated for simplicity) immediately underneath the plasma membrane, followed by dense intricate cortical actin networks organized by abLIM1 and other actin crosslinkers. For illustration purpose, the two types of cortical cytoskeleton networks are shown separately. Please see discussion for details

Back to article page