Fig. 6: Simultaneous mutations in all four CLSYs eliminate DNA methylation at RdDM target loci.

a Methylation levels of CG, CHG, and CHH at clsy1234-specific, nrpd1-specific, and their common hypo-DMRs. b Heatmap showing the methylation levels in various clsy mutants at nprd1 hypo-DMRs in different cytosine contexts (CG, CHG, and CHH). c Analysis of DNA methylation in the indicated genotypes at IGN5, AtSN1, and Solo-LTR by chop-PCR. Undigested DNA served as the control