Fig. 2: CFO increases phenotypic and functional human HSPCs.
From: Maintenance of human haematopoietic stem and progenitor cells in vitro using a chemical cocktail
a Cell morphology and numbers. Numbers of human CD34-positive cells exposed to C, F, O and CFO were calculated (scale bar, 50 μm). *P < 0.05, **P < 0.01, ***P < 0.001. Data represents the mean ± SD. b Relative expression of CD34. Human CD34-positive cells exposed to C, F, O and combination were assayed for relative expression of CD34. **P < 0.01. Data represents the mean ± SD. c Clone numbers. Human CD34-positive cells exposed to C, F, O and combination were assayed for colony-forming units. G, granulocyte; E, erythroid; M, myeloid; GEMM, granulocyte, erythroid, monocyte and megakaryocyte colonies. **P < 0.01. Data represents the mean ± SD. d Phenotype of HSPC. Human CD34-positive cells exposed to C, F, O and combination were assayed for surface protein expression of CD34. 7-AAD was assayed for dead cells. C, CHIR-99021; F, Forskolin; O, OAC1
