Fig. 2: eIF4GI interacts with G3BP.
From: SG formation relies on eIF4GI-G3BP interaction which is targeted by picornavirus stress antagonists
a–b HeLa cells were transfected with eIF4GI-HA for 24 h, and cell lysates were subjected to IP/MS with anti-HA antibody. Immunoprecipitates were separated via SDS-PAGE and analyzed via silver staining (a) or WB (b).The anti-G3BP antibody, which used to detect endogenous G3BP, can recognize both G3BP1 and G3BP2. c To detect the interaction of G3BP or TIA-1 with endogenous eIF4GI, HeLa cells expressing Flag-tagged G3BP1 or TIA-1 were lysed and subjected to IP with anti-Flag antibody, followed by WB to resolve the immune complexes. d–e Interaction examination between endogenous eIF4GI and G3BP (TIA-1 was used as negative control). HeLa cells were lysed and subjected to IP with antibodies against eIF4GI (d) or G3BP (e). Lysates and immunoprecipitates were resolved via WB with indicated antibodies. f HeLa cells were lysed and treated with RNaseA ( + ) or mock-treated (−) before IP with anti-G3BP antibody, followed by detection of eIF4GI and G3BP via Western blots. See also Supplementary Fig. S2
