Fig. 3: eIF4GI-G3BP interaction is required for eIF4GI assembly into tSGs.
From: SG formation relies on eIF4GI-G3BP interaction which is targeted by picornavirus stress antagonists
a Graphic description of eIF4GI and its mutants. b–f eIF4GI-HA and its mutants were expressed as indicated. Cell lysates were subjected to IP and analyzed as in Fig. 2b (b and d). Cells were treated with 200 μM AS for 1 h, then fixed and stained with HA (purple), G3BP (red), and TIA-1 (green). G3BP and TIA-1 served as indicators of tSGs (c and e). Quantitation of SGs containing indicated HA foci in c and e (f). n = 3, 300 cells/assay were counted, mean ± SD, N.D., not detectable. Scale bars, 10 μm. Supplementary Fig. S3
