Fig. 5: RBD of G3BP is required for eIF4GI-G3BP interaction.
From: SG formation relies on eIF4GI-G3BP interaction which is targeted by picornavirus stress antagonists
a Graphic description of G3BP and its mutants. b and c HeLa cells were transfected as indicated, and cell lysates were processed as in Fig. 2c. d–e HeLa cells were transfected as indicated, treated with AS (200 μM, 1 h), and stained with TIA-1 (green) and G3BP (red) to visualize the tSGs and flag (purple) to visualize the expression of G3BP and its mutants (d). Quantitation of Flag-positive cells with TIA-1-marked tSGs (e). n = 3, 240 cells/condition were counted, mean ± SD; ***p < 0.001, n.s. no statistical significance. f–g HeLa cells transfected as indicated, and cell lysates were processed as in (b). Scale bars, 10 μm
