Fig. 7: Disruption of eIF4GI-G3BP interaction by 2A/L to block tSG formation is common among picornaviruses.
From: SG formation relies on eIF4GI-G3BP interaction which is targeted by picornavirus stress antagonists
a and b eIF4GI-HA- and eIF4GIG689E-HA-HeLa cells were transfected with 2A of EV71-BrCr, CVA, or PV for 24 h, and cells were processed as in Fig. 6a (a) and quantified as in Fig. 6b (b). (c) eIF4GIG689E-HA-HeLa cells were transfected with 2A of EV71-BrCr, CVA, or PV for 24 h and analyzed as in Fig. 6d. (d) HeLa cells were transfected with LEMCV for 24 h, then treated with 200 μM AS or not. Cells were stained with Sam68 (green) to visualize expression of LEMCV and G3BP (red) to visualize tSGs. e Quantitation of LEMCV-expressing cells with tSGs in (d). n = 3, 240 cells/condition were counted, mean ± SD; ***p < 0.001. f eIF4GI-HA-HeLa cells were transfected with LEMCV for 24 h, and cell lysates were subjected to IP as in (b). “ + ” indicates 2A or LEMCV-expressing cells. Scale bars, 10 μm
