Fig. 2: The effects of JNK-IN-8 on cell expansion.

a Representative flow plots of phenotypically defined cell subsets after 10-day culture of 10,000 fresh CD34⁺ cells supplemented with DMSO or J8 (2 μM) (n = 3 experiments). b Fold expansion of indicated phenotypically defined cell population after 10-day culture of 10,000 fresh CD34⁺ cells supplemented with DMSO or J8 (2 μM) (n = 3 experiments). TCC, total cell count. c CFU number of progenies from 1,000 day 0 CD34⁺ cells after 10-day culture supplemented with DMSO or J8 (2 μM) (n = 3 experiments). G, CFU-granulocyte; M, CFU-macrophage; GM, CFU-granulocyte and macrophage; CFU-E CFU-erythrocyte; BFU-E, erythroid burst-forming unit; GEMM, CFU-granulocyte, erythroid, macrophage, and megakaryocyte. All data shown as mean values ± SD. Statistical significance was assessed using unpaired t test, where *p < 0.05, **p < 0.01, and ****p < 0.0001; ns, not significant. See also Supplementary Fig. S1 and Table S2