Fig. 5: JNK-IN-8-induced CD34⁺ cell expansion acts by inhibiting c-Jun.

a Relative mRNA expression of indicated JNK-related genes on day 5, CD34⁺ cells cultured with DMSO or J8 (n = 3 experiments). b Western blot analysis of inhibition of phosphorylated c-Jun for DMSO and J8-treated CD34⁺ cells following serum stimulation for 30 min. c Representative flow cytometry profiles of CD34⁺ cells 5 days after transduction with lentivirus expressing shRNAs targeting c-Jun or scrambled shRNAs (n = 3 experiments). d Total cell number of CD34⁺CD45RA^- cell population in indicated cultured CD34⁺ cells on day 5 posttransfection (n = 3 experiments). e CFU numbers of 5, 000 cells on day 5 after transduction with lentivirus expressing shRNAs targeting c-Jun or scrambled shRNA (n = 3 experiments). G, CFU-granulocyte; M, CFU-macrophage; GM, CFU-granulocyte and macrophage; CFU-E, CFU-erythrocyte; BFU-E, erythroid burst-forming units; GEMM, CFU-granulocyte, erythroid, macrophage, and megakaryocyte. See also Table S4A. Sh-ctrl, CD34⁺ cells transfected with scrambled shRNA; Sh-J1, CD34⁺ cells transfected with 1# c-Jun shRNA; Sh-J2, CD34⁺ cells transfected with 2# c-Jun shRNA. All data shown as mean values ± SD. Statistical significance was assessed using unpaired t-test, where *p < 0.05; **p < 0.01, and ***p < 0.001; ND, not detected. See also Supplementary Fig. S3