Fig. 1: Binding specificity and affinity of the mAbs to ZIKV E protein. | Cell Discovery

Fig. 1: Binding specificity and affinity of the mAbs to ZIKV E protein.

From: A new class of broadly neutralizing antibodies that target the glycan loop of Zika virus envelope protein

Fig. 1

a, b Reactivities of anti-ZIKV mAbs (3E8, 5F8, 5G3, 8A2, 9C3) to the E protein ectodomains (E80) of ZIKV (a) and of DENV2 (b) measured by ELISA. Anti-EV71 mAb D5 was used as a negative control. Anti-DENV mAb D1-11 served as a positive control for detection of DENV2 E80 protein. Error bars represent standard error of the mean (SEM). c Flow cytometry analysis of reactivities of the mAbs with ZIKV and DENV viruses. ZIKV/SZ-WIV01- and DENV2/NGC-infected Vero cells were incubated with anti-ZIKV mAbs, negative control mAb D5 or flavivirus cross-reactive mAb 4G2 (positive control), and then analyzed by flow cytometry. d Binding affinities of the mAbs towards ZIKV E80 determined by biolayer interferometry (BLI). KD, equilibrium dissociation constant between the antibody and its antigen. Conc. is the abbreviation for concentration.

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