Fig. 1: Cryo-EM structure of human CALHM5 channel.
From: Cryo-EM structures of human calcium homeostasis modulator 5
a Selected 2D averages of CALHM5 in detergent. b Selected 2D averages of CALHM5 in lipid nanodiscs. c, d The cryo-EM density map of CALHM5 viewed parallel to the plasma membrane (c) or from the extracellular side down the symmetry axis (d). e–g Cartoon representation of the CALHM5 atomic model viewed in the same direction as in c (f) or d (g), or from the cytoplasmic side up the symmetry axis (e). h Cartoon representation of the CALHM5 protomer structure and schematic representation of the CALHM5 topology. i Cartoon representation of the channel pore with substrate conductive path displayed in gray sticks. j The pore radius along the substrate conductive path calculated by HOLE program1. k Two views of cartoon representation of the CALHM5 protomer structure with 5 phospholipids highlighted in stick. l Zoomed-in view of the red box region in i with hydrophobic residues shown in stick and labeled. m Schematic illustration of hypothetical CALHM5 structure in vivo in the closed state.
