Fig. 6: Dimerization of AFF1/4 is essential to Tat-dependent HIV-1 proviral transactivation. | Cell Discovery

Fig. 6: Dimerization of AFF1/4 is essential to Tat-dependent HIV-1 proviral transactivation.

From: Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription

Fig. 6

a, b Top boxes: luciferase activities were measured in NH1 cell extracts, in which wild-type or mutated Flag-AFF4/1 was transfected as indicated. The activity in the control groups were artificially set to “1.0.” The error bars represent mean +/– SD. Bottom boxes: western blot analysis of the levels of tested proteins in the transfected NH1 cells as indicated. WT, F1014A, Y1096A, 2A, and S1025A in a represent wild-type AFF4, AFF4 F1014A, AFF4 Y1096A, AFF4F1014+Y1096A, and AFF4S1025A, respectively. AFF1–4A in b represents AFF1F1063A+Y1145A+V1146A+V1152A. c, d Nuclear extracts (NE) were prepared from NH1 cells transfected with the indicated plasmids and subjected to immunoprecipitation (IP) with anti-Flag AFF4 (c) or anti-Flag AFF1 (d). The NE inputs and IP eluates were examined by immunoblotting for the presence of the tested proteins indicated on the left. The labels in c, d are same to that in a, b.

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