Fig. 1: Comparison of AP endonuclease activities of AsfvAP and EcNfo.
From: A unique DNA-binding mode of African swine fever virus AP endonuclease
a Sequence alignment of AsfvAP and homologous proteins. b Impacts of pH on the AP endonuclease activity of AsfvAP. c Comparison of the Kobs values of AsfvAP under different pH. d SDS-PAGE gel analysis showing DNA cleavage by AsfvAP under pH 6.3. e Impacts of pH on the AP endonuclease activity of EcNfo. f Comparison of the Kobs values of EcNfo under different pH. g SDS-PAGE gel analysis showing DNA cleavage by EcNfo under pH 8.0. FAM-labeled DNA-3 (5′-GGTAAGGGCAGCGTCCFCGACGAGGAATGCA-FAM-3′, 3′-CCATTCCCGTCGCAGGGGCTGCTCCTTACGT-5′) was used in all AP endonuclease assays. The substrate and product bands are labeled by S and P, respectively. DNA marker (5′-FCGACGAGGAATGCA-FAM-3′) is labeled as M. In panels b, c and e, f, the data represent the mean of three independent experiments. The standard deviation (±SD) values are indicated by error bars. All SDS-Page gel analysis were repeated for at least three times, the representative gels were shown in panels d, g.
