Fig. 5: Verification of the functional importance of R1 and R2 regions.
From: A unique DNA-binding mode of African swine fever virus AP endonuclease
a Comparison of DNA binding by WT and mutated AsfvAP. b, c SDS-PAGE gel analysis and comparison of DNA-3 cleavage by WT and mutated AsfvAP. The substrate and product bands are labeled by S and P, respectively. d Overall fold of the AsfvAP/DNA-2 complex. The two AsfvAP molecules are colored in green and cyan, respectively. The R1 regions are colored in red. dA:dG mispair is highlighted by spheres. e Superposition of the R1 regions observed in the AsfvAP/DNA-1 and AsfvAP/DNA-2 complexes. For the AsfvAP/DNA-1 complex, DNA and the R1 region are colored in orange and yellow, respectively. For the AsfvAP/DNA-2 complex, the R1 regions are colored cyan and green. The Cys16-Cys20 disulfide bonds are highlighted by sticks in both complexes. In panels a and c, the data represent the mean of three independent experiments. The standard deviation (±SD) values are indicated by error bars. All SDS-Page gel analysis were repeated for at least three times, the representative gels were shown in panel b.
